Metabolomics

Metabolomics studies focus on the analysis of small molecules (MW<2000) in samples such as, for example, tissue, body fluids, faeces, or even breath, i.e. emitted volatiles, from micro-organisms, fungi and plants to animals and humans. Large batches of samples have to be analyzed to allow for statistical differentiation between sample types, for example, from healthy humans and humans suffering from a specific disease or from diet related health issues. Achieving outstanding repeatability and reproducibility of the entire analytical workflow, in particular of every sample preparation step, is an extremely important precondition for reaching robust differentiation.

A wide range of sample preparation steps used in metabolomics analysis can be automated with the GERSTEL Multi Purpose Sampler (MPS). These include liquid handling, such as accurate dispensing by volume and/or weight, of a sample, standard, diluent or reagent. Liquid additions can be combined with high energy mechanical or ultrasound agitation, centrifugation, filtration, thermostating and liquid or solid phase extraction and cleanup techniques.

The complete workflow is controlled by the GERSTEL Maestro software further increasing productivity by parallel processing of multiple samples, optimized for throughput and repeatability. When samples are treated in an identical manner, the time span between sample preparation and injection to the analysis system is the exact same for all samples. Typically a prepared sample should be injected immediately after the sample preparation has been completed for optimum accuracy and repeatability. By reading and processing barcodes, the MPS can make the process even more convenient, traceable, and error proof.

When processing samples with matrix or derivatization reagent residues that can contaminate and clog up the GC-MS(/MS) system, the GERSTEL Automated Liner Exchange System (ALEX) enables the removal of built-up residue in the GC-inlet by automatically replacing the inlet liner of the GERSTEL Cooled Injection System (CIS). ALEX can therefore replace costly and labor intensive cleanup processes while safeguarding throughput 24/7. ALEX has been in routine use in major contract laboratories for well over a decade.