The determination of drugs of abuse in dried blood spots (DBS) has become increasingly popular due to several advantages associated with sample collection and storage compared to other biological matrices. The extraction of dried blood spots typically involves manual intervention. First, a small disc is punched out of the center of a dried blood spot card. Following solvent extraction of the sample, it is also common to include further cleanup using liquid-liquid extraction (LLE) to improve detection limits or exchange solvents for compatibility with subsequent chromatographic separations. Modern analytical labs are looking to automation to help reduce solvent usage and increase sample throughput while ensuring the high quality of the resulting data.