Double Consecutive Stir Bar Sorptive Extraction (D-SBSE) Analysis for Total Extraction Calculation

Accurate quantification of VOCs/SVOCs in complex matrices is critical, but traditional extraction techniques face limitations like inconsistent compound extraction efficiencies and strong matrix effects. This study introduces a double consecutive stir bar sorptive extraction (D-SBSE) method for exhaustive extraction of odor-active compounds in aqueous samples, evaluated against four consecutive SBSE extractions.

The method was found to enhance efficiency via theoretical total peak area calculation by extrapolation, using double consecutive extraction data integration. Experimental optimization of extraction time (2 h for NaCl solutions, 4 h for aqueous solutions) and salt addition (NaCl) enhanced partition coefficients via salting-out effects.

Comparative analysis showed that D-SBSE achieved relative deviations comparable to total extraction (0.02–21.53% in NaCl solutions, 0.19–29.79% in aqueous solutions). The method overcame matrix dependency by integrating dual extraction data-enabling robust quantification with simplified workflow and improved throughput.