Stir Bar Sorptive Extraction (SBSE) is an innovative and efficient method for the extraction of drugs and pharmaceuticals from blood-, urine- and tissue samples in a forensic toxicology laboratory. As shown in this application note and earlier publications SBSE is an effective screening tool for drugs and pharmaceuticals in biological fluids and tissue. The SBSE technique is easy to use and the method described in this publication is performed without additional use of organic solvents, centrifugation, or the liquid transfer steps that are normally necessary when performing liquid-liquid or solid-phase extraction.

GC/MS determination of pharmaceutical compounds and drugs in biological samples is usually done by isolating the compounds of interest and/or their metabolites from the biological matrix. The sample preparation techniques most often used in routine analysis are based on liquid-liquid extraction, protein precipitation and solid-phase extraction (SPE).